2019-05-08 09:43:572025-05-20 16:12:05
locus
BSU04320
BSU_04320
product
high-affinity potassium transporter
high-affinity K+/H+ symporter
outlinks
bsu
BSU04320
BSU_04320
The protein
Catalyzed reaction/ biological activity
uptake of potassium [pubmed|28420751]
K+/H+ symport [pubmed|32005818,28420751]
The protein
Protein family
[SW|amino acid-polyamine-organocation (APC) superfamily] (according to UniProt)
KUP family (single member) [pubmed|32005818]
[SW|amino acid-polyamine-organocation (APC) superfamily] (according to UniProt)
The protein
[SW|Domains]
11 trans-membrane helices at the N-terminus [pubmed|28420751]
12 trans-membrane helices at the N-terminus [pubmed|32005818]
C-terminal cytoplasmic domain [pubmed|28420751]
Biological materials
Mutant
MGNA-C090 (kimA::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/2088 NBRP B. subtilis, Japan]
1A688 (''[[gene|kimA]]''::''erm''), available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A688&Search=1A688 BGSC]
GP93 ([[gene|kimA]]::''cat''), available in [SW|Jörg Stülke]'s lab [pubmed|28420751]
JH642 and its derivatives: natural deletion of the 18 kb [[gene|ydzA]]-[[gene|mntH]] region encompassing the genes [[gene|ydzA]]-[[gene|topB]]-[[gene|ydaJ]]-[[gene|ydaK]]-[[gene|ydaL]]-[[gene|ydaM]]-[[gene|ydaN]]-[[gene|kimA]]-[[gene|mutT]]-[[gene|ydaP]]-[[gene|ydzK]]-[[gene|mntH ]][pubmed|18670626]
BKE04320 ([[gene|kimA]]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE04320 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATCGATGTCTTCCCCTTTT, downstream forward: _UP4_TAAAGCTCTAGGACCAAGGG
BKK04320 ([[gene|kimA]]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK04320 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATCGATGTCTTCCCCTTTT, downstream forward: _UP4_TAAAGCTCTAGGACCAAGGG
GP2720 ([[gene|kimA]]::''spc''), available in [SW|Jörg Stülke]'s lab
GP2721 ([[gene|kimA]]::''ermC''), available in [SW|Jörg Stülke]'s lab
GP2498 ([[gene|kimA]]::''cat'' [[gene|ktrA]]-[[gene|ktrB]]::''spc''), available in [SW|Jörg Stülke]'s lab
MGNA-C090 (kimA::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/2088 NBRP B. subtilis, Japan]
1A688 (''[[gene|kimA]]''::''erm''), available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A688&Search=1A688 BGSC]
GP93 (Δ[[gene|kimA]]::''cat''), available in [SW|Jörg Stülke]'s lab [pubmed|28420751]
JH642 and its derivatives: natural deletion of the 18 kb [[gene|ydzA]]-[[gene|mntH]] region encompassing the genes [[gene|ydzA]]-[[gene|topB]]-[[gene|ydaJ]]-[[gene|ydaK]]-[[gene|ydaL]]-[[gene|ydaM]]-[[gene|ydaN]]-[[gene|kimA]]-[[gene|mutT]]-[[gene|ydaP]]-[[gene|ydzK]]-[[gene|mntH ]][pubmed|18670626]
BKE04320 (Δ[[gene|kimA]]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE04320 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATCGATGTCTTCCCCTTTT, downstream forward: _UP4_TAAAGCTCTAGGACCAAGGG
BKK04320 (Δ[[gene|kimA]]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK04320 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATCGATGTCTTCCCCTTTT, downstream forward: _UP4_TAAAGCTCTAGGACCAAGGG
GP2720 (Δ[[gene|kimA]]::''spc''), available in [SW|Jörg Stülke]'s lab
GP2721 (Δ[[gene|kimA]]::''ermC''), available in [SW|Jörg Stülke]'s lab
GP2498 (Δ[[gene|kimA]]::''cat'' [[gene|ktrA]]-[[gene|ktrB]]::''spc''), available in [SW|Jörg Stülke]'s lab
labs
[SW|Jörg Stülke], Göttingen, Germany
[SW|Jörg Stülke], University of Göttingen, Germany [http://genmibio.uni-goettingen.de Homepage]
[SW|Inga Hänelt], Frankfurt, Germany [https://www.biochem.uni-frankfurt.de/index.php?id=298 Homepage]
References
Reviews
References
Original publications
The protein
Kinetic information
KM: 215 µM [pubmed|32005818]
Vmax 245 nmol min−1 mg−1 [pubmed|32005818]
The protein
Effectors of protein activity
activity is inhibited upon binding of c-di-AMP [pubmed|31061098]
in the Listeria monocytogenes protein, c-di-AMP binds to the C-terminal cytoplasmic domain of KimA [pubmed|31506295]
The protein
Structure
[PDB|6S3K] [pubmed|32005818]